DNA sequences of a eukaryotic gene that one should introduce in a host cell to have full expression?

State at least five DNA sequences of a eukaryotic gene that one should introduce in a host cell to have full expression of that foreign gene in a bacterial host cell.



I'm totally confused by what this question is asking for?? Can any one help me figure this out?DNA sequences of a eukaryotic gene that one should introduce in a host cell to have full expression?
This may be more advanced then you need but:



1. You'll need a plasmid with your gene in it. A plasmid acts like it's own chromosome so it won't be lost in the cell. You can insert your gene of interest into the plasmid and insert this into a eukaryotic cell.



2. You'll need a promoter to enhance mRNA transcription.



3. You'll need a Kozak consensus sequence that is needed for eukaryotic mRNA translation.



4. You'll need the start site which is the indicator of where protein translation should start.



5. You'll need a stop site which is the indicator of where protein translation should end.DNA sequences of a eukaryotic gene that one should introduce in a host cell to have full expression?
You were asked to mention what are the main sequences needed for mRNA transcription from DNA. (Full expression means protein synthesis right?)

What I know for sure is promoter. DNA can't be transcript without it. You may search for the other, or maybe someone else know for sure.