What is the best way to extract genomic DNA out of staphyloccocus bacteria?

Ive tried with a DNA mini prep kit using lysozyme, but it didn't work, the kit suggests using lysostaphin, but its expensive, anyother suggestions?What is the best way to extract genomic DNA out of staphyloccocus bacteria?
When I was working in the biochemistry lab, I was using sonication first to lyse the bacteria. I don't know for certain about your case, but the lysozyme wasn't strong enough for for my bacteria. I used lysozymes for the larger, less durable mammalian cells.What is the best way to extract genomic DNA out of staphyloccocus bacteria?
I don't think you can use a mini prep to get out genomic DNA. Mini preps are for plasmids which are much small then genomic DNA. In yeast we do a phenol-chloroform extraction to get out genomic DNA. Try doing a pubmed search for someone who isolated genomic DNA from staph.What is the best way to extract genomic DNA out of staphyloccocus bacteria?
A miniprep kit is designed to isolate plasmid DNA, so it will not work for genomic DNA. The genomic DNA is much larger and gets stuck in that glop when you add the neutralization solution. There are probably genomic DNA kits out there, so you might search for those. An old school method for separating plasmid DNA from genomic DNA is cesium chloride centrifugation. When working with genomic DNA it is important to be gentle (ie don't vortex) because the DNA can shear into pieces.

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