What is the benefit of cells suspension in DNA seperation protocol?

I read a biology article describing protocol for seperating DNA using electrophoresis method such as PAGE and PFGE-CHEF.

They mentioned "cell suspension" more than once, but I couldn't understand what that means.



Does it mean killing the cells? causing them lysis?

Or does it mean just stopping them from moving?

And why is it important to do it?



Thanks.What is the benefit of cells suspension in DNA seperation protocol?
Yeah, you have to lyse the cells, and to get good lysis you want to have the cells well suspended. That way when you extract the DNA each cell is well exposed to the chemical treatment you use. You would not want the cells in a pellet at the bottom of a tube and to just treat the top layer of cells.



And why lyse them? Because if you want to run the DNA on a gel then it has to be purified. That means getting rid of the proteins and lipids and all the other stuff. BTW, you can use different kinds of treatments on cells and extract just proteins, or lipids or DNA. It just depends on the kind of treatment that you use.