Why is it important to use cDNA in making recombinant DNA from a mammal to be expressed in a bacterial culture?
What is a DNA fingerprint and how is it obtained and used?
How is the p53 gene involved in healthy cells and how does this help us kill tumor cells?
Please help!Why is it important to use cDNA in making recombinant DNA from a mammal to be expressed in a bacterial culture?
You use cDNA as this is produced from the mRNA of the mammal. The mRNA has had all the introns spliced out of it, and this is important as bacteria do not splice and so if you just put in regular DNA the protein would not form right.
A DNA fingerprint is a unique way of identifying each persons DNA by looking at markers determined throughout the molecule. It can be used in paternity tests and for catching criminals
p53 is a tumour supressor and stops the uncontrolled divison of cells seen in tumours. in something crazy like 80% of cancer the p53 gene is mutated and so by cloning by complimentation it is possible to restore the p53 function and prevent the tumour from proliferating
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