Sunday, March 4, 2012

What is the difference between a Klenow fragment and DNA Polymerase III?

So apparently Klenow fragment is basically DNA Pol I without the 5- to 3- exonuclease activity. It retains its 3' to 5' exonuclease activity and its 5' to 3' polymerase activity. In research, could you replace Klenow fragment by DNA Pol III?What is the difference between a Klenow fragment and DNA Polymerase III?
Klenow fragment is the name given to the 75kd alpha subunit of DNA Polymerase I from E. coli. It has it retains the 5'-3' polymerase synthesis activity and the 3鈥?鈫?5鈥?exonuclease activity for removal of precoding nucleotides and proofreading, but loses its 5' 鈫?3' exonuclease activity.



It does seem that Klenow and Pol III have similiar capabilities, but it looks like Pol III is faster at synthesis than Klenow.

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